Our TIRF microscope is a Nikon Ti Eclipse with the following equipment: Blue (488 nm, 20 mW) and green (532 nm, 100 mW) solid state lasers Intensitylight (white light source) CoolLED (400, 465 and 525 nm, diod light source) Port for TIRF illumination with white light Port for TIRF illumination with laser light through fiber entrance Perfect focus system 60x oil objective for TIRF 60x water objective with long working distance (~2 mm) 20x air objective 10x air objective 4x air objective TRITC and FITC filter cubes Andor Ixon DU-987E cooled CCD camera